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Image Search Results
Journal: Biomedical Optics Express
Article Title: Targeted photothrombotic subcortical small vessel occlusion using in vivo real-time fiber bundle endomicroscopy in mice
doi: 10.1364/BOE.473407
Figure Lengend Snippet: Schematic of entire FBEµ including a control system for real-time fluorescence imaging and laser patterned photothrombosis. (A) System schematic diagram of FBEµ. It combined a fiber bundle probe, a dual-color fluorescence imaging system, and a 532-nm laser patterned system. (B) The CCD camera and 532-nm laser were synchronized and controlled by the digital delay pulse generator. Control signal timing was relative to the delay zero point (T0) of the digital delay pulse generator internal trigger. The CCD camera ran on every output pulse (T1) of the digital delay pulse generator. Also, the shutter in front of the broadband light source was controlled by the CCD’s exposure TTL signal output, which opened the shutter when the camera was exposed. The digital delay pulse generator output pulses (T2) triggered a 532-nm laser. (C) Dual-color raw fluorescence image of the central indicator pattern obtained from FBEµ using green fluorescence reference slide. The dual-color image was the same sample fluorescence image just optically split using a dichroic mirror 2 into two emission ranges: 520 nm to 550 nm (top left, green fluorescence) and 573 nm to 613 nm (bottom right, red fluorescence). (D) Cropped, magnified, and merged images inside the white boxes in Figure C after interpolation to remove pixelation. The upper-left and lower-right box images are shown in green and red pseudo-colors, respectively. (E) Cropped and magnified green fluorescence image of an interpolated 532-nm laser circular pattern image. The top center represents the laser pattern diameter. Lens 1: f = 180 mm; Lens 2: f = 150 mm; Lens 3: f = 75 mm; Lens 4: f = 180 mm; Dichroic mirror 1: XF2041; Dichroic mirror 2: 660dclp; Ex filter: 479-25/585-25; Em. Filter 1: 525/50Em.; and Em. Filter 2: 593/40Em. Abbreviations: SLM: spatial light modulator and PBS: polarizing beam splitter. Scale bars, 50 µm.
Article Snippet: Finally, the fiber bundle endomicrosopic image was reconstructed using
Techniques: Control, Fluorescence, Imaging